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iFluor® 647 goat anti-rabbit IgG (H+L)
Cross Adsorbed
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 647 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~654 nm and ~674 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 647 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;647 goat anti-rabbit IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>
<p>HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;647 goat anti-rabbit IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16710200 ug
Price
168371 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on September 23, 2025