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AAT Bioquest

iFluor® 660 Anti-human CD23 Antibody *EBVCS-5*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)663
Emission (nm)678
Quantum yield0.261
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
FcεRII; B6; BLAST-2; Leu-20; Low affinity IgE receptor
Clone
EBVCS-5
Host
mouse
Isotype
Mouse IgG1, κ
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.07
Correction Factor (280 nm)
0.08
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
663
Emission (nm)
678
Quantum yield
0.261
EBVCS-5 is an anti-human antibody that forms an immune complex with the CD23 antigen. CD23 (sometimes referred to as FcεRII, BLAST-2 or B6) is a glycoprotein that is expressed on the surface of cells like platelets, granulocytes, T cells, dendritic cells and epithelial cells. CD23 is a component of important cellular pathways, for example, the cytokine-mediated signaling pathway and Notch signaling pathway. Furthermore, in certain organisms, it is a promoter of nitric-oxide synthase activity, acts to positively regulate killing of cells of other organism and positively regulates nitric-oxide synthase biosynthetic process. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like CD11b, CD21, IgE and CD11c. CD23 is a fairly uncommon antibody target, with a little more than 5800 publications in the last decade. Even still, CD23 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of . This antibody was purified through affinity chromatography and conjugated to iFluor® 660 (ex/em = 663/678 nm). It is compatible with the 642 nm laser and 664/20 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)663
Emission (nm)678
Quantum yield0.261

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd23 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.