Name: iFluor® 700 Anti-human CD14 Antibody *HI221*
Brand: AAT Bioquest
SKU: 101400J0
Availability: InStock

iFluor® 700 Anti-human CD14 Antibody

HI221

HI221 is an anti-human monoclonal antibody that is specific for the CD14 antigen. CD14 (sometimes referred to as myeloid cell-specific leucine-rich glycoprotein or LPS receptor) is a glycoprotein that is found on the surface of cells like granulocytes and macrophages. CD14 plays a role in vital cellular pathways, namely, the apoptotic signaling pathway, lipopolysaccharide-mediated signaling pathway and cell surface receptor signaling pathway. In addition, in many organisms, it enhances interleukin-8 secretion, acts to positively regulate interferon-gamma production and positively regulates NIK/NF-kappaB signaling. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as Endotoxin and TLR4. CD14 is a very popular antibody target, with over 42000 publications in the last decade. CD14 has a variety of applications in neuroinflammation, neuroscience and cell biology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 700 (ex/em = 690/713 nm).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
101400J0	100 tests	Price
101400J1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD14 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	LPS-Receptor
Clone	HI221
Host	Mouse
Isotype	IgM
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.04
Correction factor (565 nm)	0.0766
Correction factor (650 nm)	0.4004
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	690
Emission (nm)	713
Quantum yield	0.23 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 22, 2026