Name: iFluor® 700 Anti-human CD40 Antibody *G28.5*
Brand: AAT Bioquest
SKU: 104010J0
Availability: InStock

iFluor® 700 Anti-human CD40 Antibody

G28.5

G28.5 is an anti-human monoclonal antibody that targets the CD40 antigen. CD40 (also known as Bp50) is a 48 kD transmembrane protein that is expressed on the surface of cells like macrophages, dendritic cells and stem cells. CD40 is a component of key cellular pathways, in particular, the tumor necrosis factor-mediated signaling pathway, immune response-regulating cell surface receptor signaling pathway and CD40 signaling pathway. Also, in many organisms, it upregulates interleukin-12 production, is a positive regulator of isotype switching to IgG isotypes and is a promoter of protein phosphorylation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like CD40L, CD154 and TRAP. CD40 is a very popular antibody target, with over 30000 publications in the last decade. CD40 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to iFluor® 700 (ex/em = 690/713 nm).

Datasheet

COA

Catalog	Size	Price	Quantity
104010J0	100 tests	Price
104010J1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD40 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	BP50, TNFRSF5
Clone	G28.5
Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.04
Correction factor (565 nm)	0.0766
Correction factor (650 nm)	0.4004
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	690
Emission (nm)	713
Quantum yield	0.23 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Purchase order	Send to sales@aatbio.com
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Page updated on August 21, 2026