Name: iFluor® 700 Anti-human CD45 Antibody *HI73*
Brand: AAT Bioquest
SKU: 104510J0
Availability: InStock

iFluor® 700 Anti-human CD45 Antibody

HI73

HI73 is an anti-human monoclonal antibody that recognizes the CD45 antigen. CD45 (also known as PTPRC, Leukocyte common antigen or T200) is a 180 - 240 kD transmembrane glycoprotein that is found on the surface of cells like stem cells, dendritic cells and B cells. CD45 has been associated with important biological processes such as dephosphorylation, especially protein dephosphorylation. Additionally, in certain organisms, it positively regulates tumor necrosis factor production, promotes stem cell proliferation and promotes isotype switching to IgG isotypes. CD45 is involved with key cellular pathways, for example, the B cell receptor signaling pathway, T cell receptor signaling pathway and negative regulation of cytokine-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as p56lck, Src kinases and p59fyn. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 has a variety of applications in cell biology and inhibitory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 700 (ex/em = 690/713 nm).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
104510J0	100 tests	Price
104510J1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD45 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Common Antigen (LCA), T200, PTPRC
Clone	HI73
Host	Mouse
Isotype	IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.09
Correction factor (280 nm)	0.04
Correction factor (565 nm)	0.0766
Correction factor (650 nm)	0.4004
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	690
Emission (nm)	713
Quantum yield	0.23 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 16, 2026