logo
AAT Bioquest

iFluor® 750 Anti-human CD279 Antibody *J116*

Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Spectral properties
Correction Factor (260 nm)0.044
Correction Factor (280 nm)0.039
Correction Factor (565 nm)0.0250
Correction Factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)2750001
Excitation (nm)757
Emission (nm)779
Quantum yield0.121
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
PD1
Clone
J116
Host
mouse
Isotype
mouse igg1, κ
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.044
Correction Factor (280 nm)
0.039
Correction Factor (565 nm)
0.0250
Correction Factor (650 nm)
0.1413
Extinction coefficient (cm -1 M -1)
2750001
Excitation (nm)
757
Emission (nm)
779
Quantum yield
0.121
The J116 monoclonal antibody binds to human CD279, a 50 - 55 kD member of the Ig superfamily commonly located on the surface of B cells and thymocytes. CD279 is associated with a variety of biologically interesting macromolecules/ligands, for instance, PDL1. CD279 is a relatively rare antibody target, with fewer than 1000 publications in the last decade. Even still, CD279 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of inhibitory molecules, cancer biomarkers and immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 750 (ex/em = 757/779 nm).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.044
Correction Factor (280 nm)0.039
Correction Factor (565 nm)0.0250
Correction Factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)2750001
Excitation (nm)757
Emission (nm)779
Quantum yield0.121

Antibody Finder


Select Your CD Antibody
Select Your Clone
Select Your Conjugate

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd279 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.