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iFluor® 750 Anti-human CD53 Antibody
HI36
The HI36 monoclonal antibody binds to human CD53, a 35 - 42 kD member of the tetraspanin/transmambrane-4 superfamily typically expressed on the surface of leukocytes, B cells, dendritic cells, osteoblasts and thymocytes. In many organisms, CD53 plays a role in the upregulation of myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, VLA-4 and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 is vital to costimulatory molecules research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 750 (ex/em = 757/779 nm).
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CatalogSize
Price
Quantity
105310L0100 tests
Price
105310L1500 tests
Price
 
Antibody properties

Other namesTetraspanin-25, MOX44
CloneHI36
HostMouse
IsotypeIgG3
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties

Correction factor (260 nm)0.044
Correction factor (280 nm)0.039
Correction factor (565 nm)0.0250
Correction factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)
275000
1
Excitation (nm)757
Emission (nm)779
Quantum yield
0.12
1
Usage and storage

Intended useResearch Use Only (RUO)
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Page updated on August 13, 2026