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iFluor® 750 Anti-human CD8 Antibody
SK1
SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. CD8a (sometimes called MAL or Leu2) is a 32 - 34 kD member of the Ig superfamily that is expressed on the surface of cells such as NK cells and T cells. CD8 is a component of important cellular pathways, for example, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 750 (ex/em = 757/779 nm).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 750 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific R7-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 750 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific R7-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
100810L0100 tests
Price
100810L1500 tests
Price
 
Antibody properties
Other namesT8, Leu2
CloneSK1
HostMouse
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Correction factor (260 nm)0.044
Correction factor (280 nm)0.039
Correction factor (565 nm)0.0250
Correction factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)
275000
1
Excitation (nm)757
Emission (nm)779
Quantum yield
0.12
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 12, 2025