iFluor® 800 Anti-human CD279 Antibody J116

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	801
Emission (nm)	820
Quantum yield	0.11¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

PD1

Clone

J116

Host

mouse

Isotype

mouse igg1, κ

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.08

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

801

Emission (nm)

820

Quantum yield

0.11¹

The J116 monoclonal antibody binds to human CD279, a 50 - 55 kD member of the Ig superfamily commonly located on the surface of B cells and thymocytes. CD279 is associated with a variety of biologically interesting macromolecules/ligands, for instance, PDL1. CD279 is a relatively rare antibody target, with fewer than 1000 publications in the last decade. Even still, CD279 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of inhibitory molecules, cancer biomarkers and immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 800 (ex/em = 801/820 nm).

Spectrum

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Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	801
Emission (nm)	820
Quantum yield	0.11¹

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd279 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780