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About
iFluor® 820 Anti-human CD43 Antibody
HI161
The HI161 monoclonal antibody reacts with human CD43, a 95 - 135 kD transmembrane protein commonly located on the surface of plasma cells, thymocytes, neutrophils, myelomas and T cells. In some organisms, CD43 plays a role in the downregulation of cell adhesion, promotes tumor necrosis factor biosynthetic process and acts to negatively regulate T cell proliferation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as EZR. CD43 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD43 has been widely used in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 820 (ex/em = 822/850 nm).
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Antibody properties

Other namesLeukocyte Sialoglycoprotein, Leukosialin, Galactoglycoprotein, SPN
CloneHI161
HostMouse
IsotypeMouse IgG1
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties

Correction factor (260 nm)0.11
Correction factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)822
Emission (nm)850
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on December 15, 2025