iFluor® 820 Anti-human/mouse CD44 Antibody *IM7*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.11 |
Correction Factor (280 nm) | 0.16 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 822 |
Emission (nm) | 850 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names ECMR-III; Pgp-1; HUTCH-1; H-CAM | Clone IM7 | Host rat | Reactivity human; mouse | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.11 | Correction Factor (280 nm) 0.16 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 822 | Emission (nm) 850 |
IM7 is an anti-human/mouse monoclonal antibody that targets the CD44 antigen. CD44 (sometimes referred to as Epican, H-CAM, PGP-1 or ECMR III) is a 85 kD glycoprotein that is found on the surface of cells like NK cells, endothelial cells and macrophages. CD44 is involved with key cellular pathways, in particular, the negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and interferon-gamma-mediated signaling pathway. Moreover, in some organisms, it upregulates peptidyl-tyrosine phosphorylation, plays a role in the upregulation of ERK1 and ERK2 cascade and is a positive regulator of peptidyl-serine phosphorylation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like MIP1β, Ankyrin, Collagen and Osteopontin. CD44 is a very popular antibody target, with over 40000 publications in the last decade. CD44 is vital to cell adhesion, cell biology and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 820 (ex/em = 822/850 nm).
Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.11 |
Correction Factor (280 nm) | 0.16 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 822 |
Emission (nm) | 850 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd44 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?