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Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*

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Physical properties
Molecular weight577.11
Spectral properties
Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Bis-Arg-rhodamine 110
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5-Carboxyrhodamine 6G maleimide
Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (47)


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Excitation (nm)
Emission (nm)
SRB sulfonyl chloride (also called Lissamine rhodamine sulfonyl chloride™) is highly fluorescent. It is quite unstable in water, especially at the higher pH required for reaction with aliphatic amines. Protein modification by this reagent is frequently done at low temperature (preferably at 4 °C). This reagent reacts with amine compounds such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable, and resistant to protease-catalyzed hydrolysis. Unlike succinimidyl esters, sulfonyl chlorides react with both aliphatic amines and aromatic amines indiscriminatingly.


Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM173.277 µL866.386 µL1.733 mL8.664 mL17.328 mL
5 mM34.655 µL173.277 µL346.554 µL1.733 mL3.466 mL
10 mM17.328 µL86.639 µL173.277 µL866.386 µL1.733 mL

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Spectral properties

Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.171
Excitation (nm)558
Emission (nm)575



View all 1 citations: Citation Explorer
Entry of 6-Residue Antimicrobial Peptide Derived from Lactoferricin B into Single Vesicles and E. coli Cells without Damaging their Membranes
Authors: Moniruzzaman, Md and Islam, Md Zahidul and Sharmin, Sabrina and Dohra, Hideo and Yamazaki, Masahito
Journal: Biochemistry (2017)


View all 22 references: Citation Explorer
One-pot fluorescent labeling of xyloglucan oligosaccharides with sulforhodamine
Authors: Kosik O, Farkas V.
Journal: Anal Biochem (2008): 232
O-oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosaccharides
Authors: Miller JG, Farkas V, Sharples SC, Fry SC.
Journal: Carbohydr Res (2007): 44
A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
Authors: Bai M, Wyatt SK, Han Z, Papadopoulos V, Bornhop DJ.
Journal: Bioconjug Chem (2007): 1118
Psoralen conjugates for visualization of genomic interstrand cross-links localized by laser photoactivation
Authors: Thazhathveetil AK, Liu ST, Indig FE, Seidman MM.
Journal: Bioconjug Chem (2007): 431
De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library
Authors: Houghten RA, Dooley CT, Appel JR.
Journal: Bioorg Med Chem Lett (2004): 1947
Fluorescent probes for monitoring virus fusion kinetics: comparative evaluation of reliability
Authors: Nunes-Correia I, Eulalio A, Nir S, Duzgunes N, Ramalho-Santos J, Pedroso de Lima MC.
Journal: Biochim Biophys Acta (2002): 65
Chemistry of sulforhodamine--amine conjugates
Authors: Corrie JE, Davis CT, Eccleston JF.
Journal: Bioconjug Chem (2001): 186
Increased liposome extravasation in selected tissues: effect of substance P
Authors: Rosenecker J, Zhang W, Hong K, Lausier J, Geppetti P, Yoshihara S, Papahadjopoulos D, Nadel JA.
Journal: Proc Natl Acad Sci U S A (1996): 7236
Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties
Authors: Lefevre C, Kang HC, Haugl and RP, Malekzadeh N, Arttamangkul S.
Journal: Bioconjug Chem (1996): 482
A comparison of direct and liposomal antibody conjugates of sulfonated aluminum phthalocyanines for selective photoimmunotherapy of human bladder carcinoma
Authors: Morgan J, Lottman H, Abbou CC, Chopin DK.
Journal: Photochem Photobiol (1994): 486