Name: mFluor™ Blue 570 Anti-human CD8 Antibody *HIT8a*
Brand: AAT Bioquest
SKU: 100800T0
Availability: InStock

mFluor™ Blue 570 Anti-human CD8 Antibody

HIT8a

The HIT8a monoclonal antibody binds to human CD8a, a 32 - 34 kD member of the Ig superfamily often located on the surface of dendritic cells, t cells and thymocytes. CD8 is a component of important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Blue 570 (ex/em = 553/565 nm). It is compatible with the 561 nm laser and 582/15 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature.
(Bottom) Flow cytometry analysis of whole blood stained with mFluor™ Blue 570 Anti-human CD8 Antibody (clone HIT8a). Fluorescence was detected using an Aurora spectral flow cytometer, specifically in the B4-A channel.

Datasheet

COA

Catalog	Size	Price	Quantity
100800T0	100 tests	Price
100800T1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD8a antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	T8, Leu2
Clone	HIT8a
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	553
Correction factor (260 nm)	0.228
Correction factor (280 nm)	0.179
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	505
Emission (nm)	564

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	553 nm
Emission	565 nm

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 5, 2026