Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

mFluor™ Red 780 Biotin Conjugate

mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near infrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near infrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.
Ordering information
Price ()
Catalog Number3115
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1671.27
SolventDMSO
Spectral properties
Absorbance (nm)630
Correction Factor (260 nm)0.101
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)629
Emission (nm)767
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Related products
mFluor™ Violet 450 SE
mFluor™ Violet 510 SE
mFluor™ Violet 540 SE
mFluor™ Blue 570 SE
mFluor™ Green 620 SE
mFluor™ Yellow 630 SE
mFluor™ Red 700 SE
mFluor™ Red 780 SE
mFluor™ Red 780 amine
mFluor™ Violet 450-VAD-FMK
mFluor™ 510-VAD-FMK
mFluor™ Violet 450-streptavidin conjugate
mFluor™ Violet 510-streptavidin conjugate
mFluor™ Violet 540-streptavidin conjugate
mFluor™ Blue 570-streptavidin conjugate
mFluor™ Green 620-streptavidin conjugate
mFluor™ Yellow 630-streptavidin conjugate
mFluor™ Red 700-streptavidin conjugate
mFluor™ Violet 450-dUTP *1 mM in Tris Buffer (pH 7.5)*
mFluor™ Violet 450 acid
mFluor™ Violet 510 acid
mFluor™ Violet 540 acid
mFluor™ Blue 570 acid
mFluor™ Green 620 acid
mFluor™ Yellow 630 acid
mFluor™ Red 700 acid
mFluor™ Violet 500 SE
mFluor™ UV375 SE
mFluor™ Red 780 acid
mFluor™ UV460 SE
mFluor™ Blue 630 SE
mFluor™ Blue 660 SE
mFluor™ Violet 610 SE
mFluor™ Green 630 SE
mFluor™ Blue 580 SE
mFluor™ Blue 590 SE
mFluor™ Blue 620 SE
mFluor™ Red 780 Maleimide
mFluor™ Violet 550 SE
mFluor™ Violet 505 SE
mFluor™ Violet 590 SE
mFluor™ Violet 545 SE
mFluor™ UV420 SE
mFluor™ UV455 SE
mFluor™ UV520 SE
mFluor™ UV540 SE
mFluor™ UV610 SE
mFluor™ Violet 450 Azide
mFluor™ Violet 450 maleimide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Violet 530 SE
mFluor™ Violet 530 maleimide
Biotin *CAS 58-85-5*
Biotin, succinimidyl ester *CAS 35013-72-0*
Biotin ethylenediamine *CAS 1217450-40-2*
Biotin cadaverine
Biotin C2 maleimide
Biotin-4-fluorescein *CAS 1032732-74-3*
Biotin hydrazide *CAS 66640-86-6*
Biotin-X NTA [Biotin-X nitrilotriacetic acid, potassium salt] *CAS 856661-92-2*
Biotin-X, succinimidyl ester *CAS 72040-63-2*
Biotin PEG2 amine *CAS 138529-46-1*
Biotin PEG2 maleimide *CAS 305372-39-8*
Biotin PEG2 succinimidyl ester
Biotin-PEG3-azide *CAS 945633-30-7*
Biotin Azide
Biotin Alkyne *CAS 773888-45-2*
Biotin PEG4 succinimidyl ester
ReadiView™ biotin acid
ReadiView™ biotin amine
ReadiView™ biotin hydrazide
ReadiView™ biotin maleimide
ReadiView™ biotin succinimidyl ester
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
Amplite® Colorimetric Biotin Quantitation Kit
Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 86303-25-5*
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 136632-31-0*
Biotin-20-dUTP *1 mM in Tris Buffer (pH 7.5)*
Cal-520®-Biotin Conjugate
Phalloidin-Biotin Conjugate
FMOC-Lys(Biotin)-OH
Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)*
Biotin PEG3 amine
Annexin V-Biotin conjugate
N6-Methyladenosine-Biotin conjugate
Biotin Styramide *Superior Replacement for Biotin Tyramide*
Cy5 biotin conjugate
Biotin-cAMP conjugate
2',3'-cGAMP-Biotin conjugate
Cy5.5 biotin conjugate
Biotin-PEG4-tyramide
HRP-Biotin Conjugate
Biotin-11-dATP
Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)*
Biotin NTA
Biotin-X IDA
ReadiCleave™ SSL biotin NHS ester
Biotin-dT Phosphoramidite
ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
ReadiLink™ Biotin Oligo and ssDNA Labeling Kit
AF532 PEG4 biotin conjugate
Biotin PEG 4 Alkyne
Cy3 biotin conjugate
Cy7 biotin conjugate
Biotin C2 Azide
Biotin Phosphoramidite
Show More (109)

OverviewpdfSDSpdfProtocol


Molecular weight
1671.27
Absorbance (nm)
630
Correction Factor (260 nm)
0.101
Correction Factor (280 nm)
0.116
Extinction coefficient (cm -1 M -1)
900001
Excitation (nm)
629
Emission (nm)
767
mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near intrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Red 780 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Red 780 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM59.835 µL299.174 µL598.347 µL2.992 mL5.983 mL
5 mM11.967 µL59.835 µL119.669 µL598.347 µL1.197 mL
10 mM5.983 µL29.917 µL59.835 µL299.174 µL598.347 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)630
Correction Factor (260 nm)0.101
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)629
Emission (nm)767

References


View all 20 references: Citation Explorer
Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020
Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476
Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636
Temperature-Responsive Hydrogel-Coated Gold Nanoshells.
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)
Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46
Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9
Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization.
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24
Genetic engineering, expression, and activity of a chimeric monoclonal antibody-avidin fusion protein for receptor-mediated delivery of biotinylated drugs in humans.
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9
Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin.
Authors: Wu, Yang and Simons, Peter C and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Analytical biochemistry (2005): 221-8
Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry.
Authors: Wu, Yang and Zwartz, Gordon and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 37-44