mFluor™ Red 780 Biotin Conjugate

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1671.27
Solvent	DMSO

Spectral properties

Absorbance (nm)	630
Correction Factor (260 nm)	0.101
Correction Factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	90000¹
Excitation (nm)	629
Emission (nm)	767

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

1671.27

Absorbance (nm)

630

Correction Factor (260 nm)

0.101

Correction Factor (280 nm)

0.116

Extinction coefficient (cm ^-1 M ^-1)

90000¹

Excitation (nm)

629

Emission (nm)

767

mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near intrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. mFluor™ Red 780 Biotin Conjugate can be used for detecting avidin, streptavidin or neutravidin conjugates. It is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mFluor™ Red 780 Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	59.835 µL	299.174 µL	598.347 µL	2.992 mL	5.983 mL
5 mM	11.967 µL	59.835 µL	119.669 µL	598.347 µL	1.197 mL
10 mM	5.983 µL	29.917 µL	59.835 µL	299.174 µL	598.347 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	630
Correction Factor (260 nm)	0.101
Correction Factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	90000¹
Excitation (nm)	629
Emission (nm)	767

Images

Figure 1. mFluor™ Red 780 Biotin Conjugate contains mFluor™ Red 780 fluorophore that is well excited by the common 350 nm laser. It is a unique color for spectral flow cytometry, and demonstrates excellent avidin binding and strong near infrared (NIR) fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers.

References

View all 20 references: Citation Explorer

Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.
Authors: Wan, Zhenzhen and Gong, Fangfang and Zhang, Mimi and He, Leiliang and Wang, Yilin and Yu, Songcheng and Liu, Jie and Wu, Yuming and Liu, Li'e and Wu, Yongjun and Qu, Lingbo and Sun, Jiaqi and Yu, Fei
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 120020

Drug-Initiated Synthesis of Heterotelechelic Polymer Prodrug Nanoparticles for in Vivo Imaging and Cancer Cell Targeting.
Authors: Vinciguerra, Daniele and Degrassi, Anna and Mancini, Laura and Mura, Simona and Mougin, Julie and Couvreur, Patrick and Nicolas, Julien
Journal: Biomacromolecules (2019): 2464-2476

Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer.
Authors: Xue, Yudong and Li, Jipeng and Yang, Guoliang and Liu, Zhiyong and Zhou, Huifang and Zhang, Weian
Journal: ACS applied materials & interfaces (2019): 33628-33636

Temperature-Responsive Hydrogel-Coated Gold Nanoshells.
Authors: Park, Hye Hun and Srisombat, La-Ongnuan and Jamison, Andrew C and Liu, Tingting and Marquez, Maria D and Park, Hansoo and Lee, Sungbae and Lee, Tai-Chou and Lee, T Randall
Journal: Gels (Basel, Switzerland) (2018)

Disulfide-based multifunctional conjugates for targeted theranostic drug delivery.
Authors: Lee, Min Hee and Sessler, Jonathan L and Kim, Jong Seung
Journal: Accounts of chemical research (2015): 2935-46

Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation.
Authors: Alexander, Seth C and Busby, Kayla N and Cole, Christian M and Zhou, Cun Yu and Devaraj, Neal K
Journal: Journal of the American Chemical Society (2015): 12756-9

Novel DNA ligase with broad nucleotide cofactor specificity from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii: influence of ancestral DNA ligase on cofactor utilization.
Authors: Sun, Younguk and Seo, Moo Seok and Kim, Jae Hyun and Kim, Yun Jae and Kim, Gun A and Lee, Jong Il and Lee, Jung-Hyun and Kwon, Suk-Tae
Journal: Environmental microbiology (2008): 3212-24

Genetic engineering, expression, and activity of a chimeric monoclonal antibody-avidin fusion protein for receptor-mediated delivery of biotinylated drugs in humans.
Authors: Boado, Ruben J and Zhang, Yufeng and Zhang, Yun and Xia, Chun-fang and Wang, Yuntao and Pardridge, William M
Journal: Bioconjugate chemistry (2008): 731-9

Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin.
Authors: Wu, Yang and Simons, Peter C and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Analytical biochemistry (2005): 221-8

Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry.
Authors: Wu, Yang and Zwartz, Gordon and Lopez, Gabriel P and Sklar, Larry A and Buranda, Tione
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 37-44