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mFluor™ Red 780 goat anti-mouse IgG (H+L) *Cross-Absorbed*

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Physical properties
Molecular weight~150 kDa
SolventWater
Spectral properties
Absorbance (nm)630
Correction Factor (260 nm)0.101
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)629
Emission (nm)767
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
~150 kDa
Absorbance (nm)
630
Correction Factor (260 nm)
0.101
Correction Factor (280 nm)
0.116
Extinction coefficient (cm -1 M -1)
900001
Excitation (nm)
629
Emission (nm)
767
mFluor™ Red 780 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ Red 780 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the contrast provided by mFluor™ Red 780 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ Red 780 is optimally excited by the red laser and emits NIR fluorescence maximally at 767 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Spectrum


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spectrum

Spectral properties

Absorbance (nm)630
Correction Factor (260 nm)0.101
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)900001
Excitation (nm)629
Emission (nm)767

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
mFluor™ Red 780 goat anti-rabbit IgG (H+L) *Cross-Absorbed*6297679000010.1010.116
mFluor™ Red 780 goat anti-rabbit IgG (H+L)6297679000010.1010.116

References


View all 9 references: Citation Explorer
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Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2014): 164-74
Tandem Dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2013)
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
Flow cytometry and the stability of phycoerythrin-tandem dye conjugates.
Authors: Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72
Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.
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Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15
A novel single molecule analysis of spontaneous and radiation-induced mutation at a mouse tandem repeat locus.
Authors: Yauk, Carole L and Dubrova, Yuri E and Grant, Gemma R and Jeffreys, Alec J
Journal: Mutation research (2002): 147-56
Near-infrared dyes for six-color immunophenotyping by laser scanning cytometry.
Authors: Gerstner, Andreas O H and Lenz, Dominik and Laffers, Wiebke and Hoffman, Robert A and Steinbrecher, Michael and Bootz, Friedrich and Tárnok, Attila
Journal: Cytometry (2002): 115-23
Allo-7: a new fluorescent tandem dye for use in flow cytometry.
Authors: Beavis, A J and Pennline, K J
Journal: Cytometry (1996): 390-5
Cy7PE and Cy7APC: bright new probes for immunofluorescence.
Authors: Roederer, M and Kantor, A B and Parks, D R and Herzenberg, L A
Journal: Cytometry (1996): 191-7