Name: mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Adsorbed*
Brand: AAT Bioquest
SKU: 49560
Availability: InStock

mFluor™ UV 375 goat anti-rabbit IgG (H+L)

Cross-Adsorbed

mFluor™ UV375 goat anti-rabbit conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards rabbit IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of rabbit primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-rabbit secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.

Datasheet

SDS

Catalog	Size	Price	Quantity
49560	200 ug	Price
49561	1 mg	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

References

View all 5 references: Citation Explorer

FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.

Authors:

Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)

A ten-color tube with dried antibody reagents for the screening of hematological malignancies.

Authors:

Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S

Journal:

International journal of laboratory hematology (2018): 136-143

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.

Authors:

De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

[THE STANDARD VALUES OF SUB-POPULATIONS OF T-HELPERS OF DIFFERENT LEVEL OF DIFFERENTIATION IN PERIPHERAL BLOOD].

Authors:

Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A

Journal:

Klinicheskaia laboratornaia diagnostika (2016): 179-84

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.

Authors:

Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31

Physical properties

Molecular weight	~150 kDa
Solvent	Water

Antibody properties

Host	Goat
Reactivity	Rabbit

Spectral properties

Absorbance (nm)	342
Correction factor (260 nm)	0.116
Correction factor (280 nm)	0.139
Extinction coefficient (cm ^-1 M ^-1)	40000 ¹
Excitation (nm)	351
Emission (nm)	387
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 29, 2026