mFluor™ UV375 Anti-human CD135 Antibody *BV10A4*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names FLT3; FLK2; STK-1 | Clone BV10A4 | Host mouse | Isotype Mouse IgG1 | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 342 | Correction Factor (260 nm) 0.099 | Correction Factor (280 nm) 0.138 | Extinction coefficient (cm -1 M -1) 300001 | Excitation (nm) 351 | Emission (nm) 387 | Quantum yield 0.941 |
BV10A4 is an anti-human monoclonal antibody that forms an immune complex with the CD135 antigen. CD135 (also known as FLT3, FLK2 or STK-1) is a 130 - 160 kD member of the immunoglobulin supergene family that is located on the surface of cells like stem cells. CD135 acts in important cellular pathways, in particular, the transmembrane receptor protein tyrosine kinase signaling pathway and cytokine-mediated signaling pathway. Also, in certain organisms, it positively regulates tyrosine phosphorylation of STAT protein, positively regulates phosphatidylinositol 3-kinase activity and is a promoter of phosphatidylinositol 3-kinase signaling. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like FL (flt3 ligand). CD135 is a relatively rare antibody target, with fewer than 900 publications in the last decade. Even still, CD135 has a variety of applications in immunology and cell biology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 379/28 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).
Spectrum
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Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd135 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?