mFluor™ UV375 Anti-human CD142 Antibody *HTF-1*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names Tissue factor; F3 | Clone HTF-1 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 342 | Correction Factor (260 nm) 0.099 | Correction Factor (280 nm) 0.138 | Extinction coefficient (cm -1 M -1) 300001 | Excitation (nm) 351 | Emission (nm) 387 | Quantum yield 0.941 |
HTF-1 is an anti-human monoclonal antibody that is specific for the CD142 antigen. CD142 (sometimes referred to as Tissue factor or F3) is a 45 kD single-pass type I membrane protein that is found on the surface of cells such as endothelial cells, epithelial cells and macrophages. CD142 has been closely linked to key biological processes like blood coagulation, specifically activation of blood coagulation via clotting cascade. Furthermore, it plays a role in vital cellular pathways, in particular, the cytokine-mediated signaling pathway, positive regulation of platelet-derived growth factor receptor signaling pathway and blood coagulation, extrinsic pathway. In certain organisms, CD142 promotes angiogenesis, upregulates endothelial cell proliferation and is involved in the positive regulation of cell migration. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like factor Xa/TFPI and factor VIIa. CD142 is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD142 is vital to angiogenesis research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 379/28 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).
Spectrum
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Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Antibody Finder
Select Your CD Antibody | |
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd142 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?