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mFluor™ UV375 Anti-human CD45 Antibody *2D1*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
Leukocyte Common Antigen (LCA); T200; PTPRC
Clone
2D1
Host
mouse
Isotype
Mouse IgG1, κ
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Absorbance (nm)
342
Correction Factor (260 nm)
0.099
Correction Factor (280 nm)
0.138
Extinction coefficient (cm -1 M -1)
300001
Excitation (nm)
351
Emission (nm)
387
Quantum yield
0.941
2D1 is an anti-human monoclonal antibody that recognizes the CD45 antigen. CD45 (also known as T200) is a 180 - 240 kD transmembrane protein that is found on the surface of cells like macrophages. CD45 has been closely linked to critical biological processes such as dephosphorylation, specifically protein dephosphorylation. Furthermore, it acts in critical cellular pathways, in particular, the positive regulation of antigen receptor-mediated signaling pathway, negative regulation of cytokine-mediated signaling pathway and regulation of receptor signaling pathway via JAK-STAT. In certain organisms, CD45 is a repressor of ERK1 and ERK2 cascade, suppresses T cell mediated cytotoxicity and downregulates protein kinase activity. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Src kinases. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 379/28 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).

Spectrum


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spectrum

Spectral properties

Absorbance (nm)342
Correction Factor (260 nm)0.099
Correction Factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)300001
Excitation (nm)351
Emission (nm)387
Quantum yield0.941

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.