Name: mFluor™ UV375 Anti-human CD99 Antibody *HIT4*
Brand: AAT Bioquest
SKU: 109910X0
Availability: InStock

mFluor™ UV375 Anti-human CD99 Antibody

The HIT4 monoclonal antibody reacts with human CD99, a transmembrane glycoprotein commonly located on the surface of epithelial cells, t cells, lymphocytes, natural killer cells and monocytes. In some organisms, CD99 positively regulates neutrophil extravasation, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, . CD99 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD99 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 379/28 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).

Protocol

COA

Catalog	Size	Price	Quantity
109910X0	100 tests	Price
109910X1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd99 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	MIC2; E2 antigen; HBA71; MSK5X
Clone	HIT4
Host	mouse
Isotype	mouse IgM
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	342
Correction factor (260 nm)	0.099
Correction factor (280 nm)	0.138
Extinction coefficient (cm ^-1 M ^-1)	30000 ¹
Excitation (nm)	351
Emission (nm)	387
Quantum yield	0.94 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 7, 2025