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mFluor™ UV375 Anti-human CD99 Antibody
HIT4
The HIT4 monoclonal antibody reacts with human CD99, a transmembrane glycoprotein commonly located on the surface of epithelial cells, t cells, lymphocytes, natural killer cells and monocytes. In some organisms, CD99 positively regulates neutrophil extravasation, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, . CD99 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD99 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV375 (ex/em = 351/387 nm). It is compatible with the 355 nm laser and 379/28 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).
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CatalogSize
Price
Quantity
109910X0100 tests
Price
109910X1500 tests
Price
 
Antibody properties

Other namesMIC2, E2 antigen, HBA71, MSK5X
CloneHIT4
HostMouse
IsotypeMouse IgM
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties

Absorbance (nm)342
Correction factor (260 nm)0.099
Correction factor (280 nm)0.138
Extinction coefficient (cm -1 M -1)
30000
1
Excitation (nm)351
Emission (nm)387
Quantum yield
0.94
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on December 21, 2025