mFluor™ UV460 Anti-human CD142 Antibody *HTF-1*

HTF-1 is an anti-human monoclonal antibody that is specific for the CD142 antigen. CD142 (sometimes referred to as Tissue factor or F3) is a 45 kD single-pass type I membrane protein that is found on the surface of cells such as endothelial cells, epithelial cells and macrophages. CD142 has been closely linked to key biological processes like blood coagulation, specifically activation of blood coagulation via clotting cascade. Furthermore, it plays a role in vital cellular pathways, in particular, the cytokine-mediated signaling pathway, positive regulation of platelet-derived growth factor receptor signaling pathway and blood coagulation, extrinsic pathway. In certain organisms, CD142 promotes angiogenesis, upregulates endothelial cell proliferation and is involved in the positive regulation of cell migration. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like factor Xa/TFPI and factor VIIa. CD142 is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD142 is vital to angiogenesis research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV460 (ex/em = 358/456 nm). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).