mFluor™ UV460 Anti-human CD28 Antibody *9.3*

The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells. CD28 is a member of vital cellular pathways, namely, the cell surface receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. Also, in some organisms, it is involved in the positive regulation of interleukin-4 production, is involved in the positive regulation of inflammatory response to antigenic stimulus and promotes isotype switching to IgG isotypes. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as PI3-kinase, CD86 and CD80. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is vital to costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV460 (ex/em = 358/456 nm). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).