mFluor™ UV460 Anti-human CD28 Antibody *CD28.2*

CD28.2 is an anti-human monoclonal antibody that forms an immune complex with the CD28 antigen. CD28 (sometimes called T44) is a 44 kD member of the Ig superfamily that is located on the surface of cells such as T cells. CD28 acts in essential cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. Furthermore, in some organisms, it positively regulates isotype switching to IgG isotypes, is an enhancer of interleukin-4 production and upregulates translation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as PI3-kinase, CD80 and CD86. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is essential for costimulatory molecules and immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV460 (ex/em = 358/456 nm). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).