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mFluor™ UV460 Anti-human CD53 Antibody *HI29*

HI29 is an anti-human monoclonal antibody that targets the CD53 antigen. CD53 (sometimes referred to as Tetraspanin-25, MOX44 or OX44) is a 35 - 42 kD member of the tetraspan family that is found on the surface of cells such as NK cells. In some organisms, CD53 enhances myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, in particular, VLA-4, Integrins and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 has a variety of applications in costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ UV460 (ex/em = 358/456 nm). It is compatible with the 355 nm laser and 447/60 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd53 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on April 25, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
105300Y0105300Y1
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesTetraspanin-25; MOX44
CloneHI29
Hostmouse
Isotypemouse IgG1
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)

364

Correction Factor (260 nm)

0.35

Correction Factor (280 nm)

0.134

Extinction coefficient (cm -1 M -1)

150001

Excitation (nm)

358

Emission (nm)

456

Quantum yield

0.861

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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