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About
mFluor™ Violet 450 Anti-human CD2 Antibody
HIT11
HIT11 is an anti-human monoclonal antibody that recognizes the CD2 antigen. CD2 (sometimes referred to as LFA-2) is a 45 kD single-pass type I membrane protein that is found on the surface of cells like T cells. CD2 has been thought to be involved with key biological processes such as cell-cell adhesion, especially heterotypic cell-cell adhesion. Also, in some organisms, it enhances myeloid dendritic cell activation, acts to positively regulate interferon-gamma secretion and is an enhancer of tumor necrosis factor production. CD2 is involved with key cellular pathways, for instance, the cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as LFA-3, CD59, CD58 and CD48. CD2 is a moderately popular antibody target, with over 16000 publications in the last decade. CD2 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 450 (ex/em = 406/445 nm). It is compatible with the 405 nm laser and 440/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 100200Z0 | 100 tests | Price | |
| 100200Z1 | 500 tests | Price |
Antibody properties
| Other names | LFA-2; Erythrocyte R; T11 |
| Clone | HIT11 |
| Host | mouse |
| Isotype | mouse IgG1 |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 406 |
| Correction factor (260 nm) | 0.338 |
| Correction factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 35000 1 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.81 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 23, 2025