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AAT Bioquest

mFluor™ Violet 450 Anti-human CD206 Antibody *15-2*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
MMR; MRC1
Clone
15-2
Host
mouse
Isotype
Mouse IgG1 kappa
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Absorbance (nm)
406
Correction Factor (260 nm)
0.338
Correction Factor (280 nm)
0.078
Extinction coefficient (cm -1 M -1)
350001
Excitation (nm)
406
Emission (nm)
445
Quantum yield
0.811
The 15-2 monoclonal antibody recognizes human CD206, a 162 - 175 kD transmembrane protein frequently expressed on the surface of endothelial cells, dendritic cells and macrophages. CD206 is associated with a variety of biologically interesting macromolecules/ligands, namely, plant glycoproteins, viral glycoproteins and lutropin. CD206 is a fairly uncommon antibody target, with a little more than 6900 publications in the last decade. Even still, CD206 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience cell markers. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 450 (ex/em = 406/445 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum


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spectrum

Spectral properties

Absorbance (nm)406
Correction Factor (260 nm)0.338
Correction Factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)350001
Excitation (nm)406
Emission (nm)445
Quantum yield0.811

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd206 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.