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mFluor™ Violet 450 Anti-human CD28 Antibody
CD28.2
CD28.2 is an anti-human monoclonal antibody that forms an immune complex with the CD28 antigen. CD28 (sometimes called T44) is a 44 kD member of the Ig superfamily that is located on the surface of cells such as T cells. CD28 acts in essential cellular pathways, in particular, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. Furthermore, in some organisms, it positively regulates isotype switching to IgG isotypes, is an enhancer of interleukin-4 production and upregulates translation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as PI3-kinase, CD80 and CD86. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is essential for costimulatory molecules and immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 450 (ex/em = 406/445 nm). It is compatible with the 405 nm laser and 440/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with mFluor™ Violet 450 Anti-human CD28 Antibody (CD28.2) (Cat. #102800Z0). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V3-A channel, demonstrating clear detection of CD28⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with mFluor™ Violet 450 Anti-human CD28 Antibody (CD28.2) (Cat. #102800Z0). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V3-A channel, demonstrating clear detection of CD28⁺ cells.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
102800Z0100 tests
Price
102800Z1500 tests
Price
 
Antibody properties
Other namesTp44, T44
CloneCD28.2
HostMouse
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Absorbance (nm)406
Correction factor (260 nm)0.338
Correction factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)
35000
1
Excitation (nm)406
Emission (nm)445
Quantum yield
0.81
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on May 5, 2026