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mFluor™ Violet 450 Anti-human CD45 Antibody
HI73
HI73 is an anti-human monoclonal antibody that recognizes the CD45 antigen. CD45 (also known as PTPRC, Leukocyte common antigen or T200) is a 180 - 240 kD transmembrane glycoprotein that is found on the surface of cells like stem cells, dendritic cells and B cells. CD45 has been associated with important biological processes such as dephosphorylation, especially protein dephosphorylation. Additionally, in certain organisms, it positively regulates tumor necrosis factor production, promotes stem cell proliferation and promotes isotype switching to IgG isotypes. CD45 is involved with key cellular pathways, for example, the B cell receptor signaling pathway, T cell receptor signaling pathway and negative regulation of cytokine-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as p56lck, Src kinases and p59fyn. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 has a variety of applications in cell biology and inhibitory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 450 (ex/em = 406/445 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the Beckman Coulter Navios EX).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 104510Z0 | 100 tests | Price | |
| 104510Z1 | 500 tests | Price |
Antibody properties
| Other names | Leukocyte Common Antigen (LCA); T200; PTPRC |
| Clone | HI73 |
| Host | mouse |
| Isotype | mouse IgG2a |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 406 |
| Correction factor (260 nm) | 0.338 |
| Correction factor (280 nm) | 0.078 |
| Extinction coefficient (cm -1 M -1) | 35000 1 |
| Excitation (nm) | 406 |
| Emission (nm) | 445 |
| Quantum yield | 0.81 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 16, 2025