mFluor™ Violet 450 Anti-mouse CD86 Antibody GL-1

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Overview SDS Protocol

Other names

B7-2; B70

Clone

GL-1

Host

rat

Isotype

Rat IgG2a kappa

Reactivity

mouse

Application

Flow Cytometry (FACS); Fluorescence Imaging

Absorbance (nm)

406

Correction Factor (260 nm)

0.338

Correction Factor (280 nm)

0.078

Extinction coefficient (cm ^-1 M ^-1)

35000¹

Excitation (nm)

406

Emission (nm)

445

Quantum yield

0.81¹

GL-1 is an anti-mouse monoclonal antibody that recognizes the CD86 antigen. CD86 (sometimes called B7-2 or B70) is a 80 kD glycoprotein that is expressed on the surface of cells such as T cells. CD86 is associated with a variety of biologically interesting macromolecules/ligands, namely, CD152 (CTLA-4). CD86 is a very popular antibody target, with over 21000 publications in the last decade. CD86 has been widely used in cell biology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 450 (ex/em = 406/445 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD FACSAria™ III).

Spectrum

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Spectral properties

Absorbance (nm)	406
Correction Factor (260 nm)	0.338
Correction Factor (280 nm)	0.078
Extinction coefficient (cm ^-1 M ^-1)	35000¹
Excitation (nm)	406
Emission (nm)	445
Quantum yield	0.81¹

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Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd86 antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780