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mFluor™ Violet 500 Anti-human CD16 Antibody
HI16a
The HI16a monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane glycoprotein often located on the surface of neutrophils, natural killer cells and macrophages. CD16 is involved with critical cellular pathways, for instance, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like IgG Fc. CD16 is a very popular antibody target, with over 25000 publications in the last decade. CD16a is essential for innate immunity research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 500 (ex/em = 410/501 nm). It is compatible with the 405 nm laser and 512/18 nm bandpass filter (for example, as in the Luminex Guava easyCyte).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10160100 | 100 tests | Price | |
| 10160101 | 500 tests | Price |
Antibody properties
| Other names | FCRIIIA |
| Clone | HI16a |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 412 |
| Correction factor (260 nm) | 0.769 |
| Correction factor (280 nm) | 0.365 |
| Extinction coefficient (cm -1 M -1) | 25000 1 |
| Excitation (nm) | 410 |
| Emission (nm) | 501 |
| Quantum yield | 0.81 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 9, 2026