Name: mFluor™ Violet 500 Anti-human CD32 Antibody *3D3*
Brand: AAT Bioquest
SKU: 10321100
Availability: InStock

mFluor™ Violet 500 Anti-human CD32 Antibody

3D3

The 3D3 monoclonal antibody binds to human CD32, a 40 kD member of the Ig superfamily often located on the surface of platelets, monocytes, B cells, granulocytes and dendritic cells. CD32 is involved with essential cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands. CD32 is a fairly uncommon antibody target, with a little more than 7000 publications in the last decade. Even still, CD32 has been widely used in innate immunity and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 500 (ex/em = 410/501 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Protocol

COA

Catalog	Size	Price	Quantity
10321100	100 tests	Price
10321101	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd32 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	FcγRII; Fc gamma RII
Clone	3D3
Host	mouse
Isotype	Mouse IgG1 kappa
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	412
Correction factor (260 nm)	0.769
Correction factor (280 nm)	0.365
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	410
Emission (nm)	501
Quantum yield	0.81 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 11, 2025