Name: mFluor™ Violet 500 Anti-human CD43 Antibody *HI161*
Brand: AAT Bioquest
SKU: 10431100
Availability: InStock

mFluor™ Violet 500 Anti-human CD43 Antibody

HI161

The HI161 monoclonal antibody reacts with human CD43, a 95 - 135 kD transmembrane protein commonly located on the surface of plasma cells, thymocytes, neutrophils, myelomas and T cells. In some organisms, CD43 plays a role in the downregulation of cell adhesion, promotes tumor necrosis factor biosynthetic process and acts to negatively regulate T cell proliferation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as EZR. CD43 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD43 has been widely used in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 500 (ex/em = 410/501 nm). It is compatible with the 405 nm laser and 510/50 nm bandpass filter (for example, as in the BD FACSCanto™ II).

COA

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd43 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Sialoglycoprotein; Leukosialin; Galactoglycoprotein; SPN
Clone	HI161
Host	mouse
Isotype	mouse IgG1
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	412
Correction factor (260 nm)	0.769
Correction factor (280 nm)	0.365
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	410
Emission (nm)	501
Quantum yield	0.81 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 5, 2025