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mFluor™ Violet 510 Anti-human CD120a Antibody *H398*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
TNFR-I; TNFRSF1A; P60; P55
Clone
H398
Host
mouse
Isotype
Mouse IgG2a
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Absorbance (nm)
410
Correction Factor (260 nm)
0.464
Correction Factor (280 nm)
0.366
Extinction coefficient (cm -1 M -1)
250001
Excitation (nm)
412
Emission (nm)
505
Quantum yield
0.861
H398 is an anti-human monoclonal antibody that is specific for the CD120a antigen. CD120a (alternatively called P55, TNFR-I, P60 or TNFRSF1A) is a 55 kD member of the TNFR superfamily that is expressed on the surface of cells such as B cells and T cells. CD120a is associated with a variety of biologically interesting macromolecules/ligands, for instance, TRAF, TRADD and ltα. CD120a is a relatively rare antibody target, with fewer than 300 publications in the last decade. Even still, CD120a is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 510 (ex/em = 412/505 nm). It is compatible with the 405 nm laser and 525/45 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).

Spectrum


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spectrum

Spectral properties

Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd120a antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.