mFluor™ Violet 510 Anti-human CD148 Antibody *MEM-CD148/05*

MEM-CD148/05 is an anti-human monoclonal antibody that forms an immune complex with the CD148 antigen. CD148 (sometimes called p260 or DEP-1) is a glycoprotein that is found on the surface of cells such as dendritic cells, platelets, B cells and NK cells. CD148 acts in key cellular pathways, for instance, the T cell receptor signaling pathway, negative regulation of epidermal growth factor receptor signaling pathway and positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis. In addition, in many organisms, it upregulates MAPK cascade, is an inhibitor of cell growth and represses cell migration. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands. CD148 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD148 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 510 (ex/em = 412/505 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD FACSymphony™ A5).