Name: mFluor™ Violet 510 Anti-human CD43 Antibody *HI165*
Brand: AAT Bioquest
SKU: 10430110
Availability: InStock

mFluor™ Violet 510 Anti-human CD43 Antibody

HI165

The HI165 monoclonal antibody reacts with human CD43, a 95 - 135 kD transmembrane protein commonly located on the surface of plasma cells, thymocytes, neutrophils, myelomas and T cells. In some organisms, CD43 plays a role in the downregulation of cell adhesion, promotes tumor necrosis factor biosynthetic process and acts to negatively regulate T cell proliferation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as EZR. CD43 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD43 has been widely used in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 510 (ex/em = 412/505 nm). It is compatible with the 405 nm laser and 528/46 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
10430110	100 tests	Price
10430111	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD43 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leukocyte Sialoglycoprotein, Leukosialin, Galactoglycoprotein, SPN
Clone	HI165
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	410
Correction factor (260 nm)	0.464
Correction factor (280 nm)	0.366
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	412
Emission (nm)	505
Quantum yield	0.86 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 11, 2026