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mFluor™ Violet 510 Anti-human CD45 Antibody *HI185*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
Leukocyte Common Antigen (LCA); T200; PTPRC
Clone
HI185
Host
mouse
Isotype
mouse IgG1
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Absorbance (nm)
410
Correction Factor (260 nm)
0.464
Correction Factor (280 nm)
0.366
Extinction coefficient (cm -1 M -1)
250001
Excitation (nm)
412
Emission (nm)
505
Quantum yield
0.861
The HI185 monoclonal antibody reacts with human CD45, a 180 - 240 kD transmembrane glycoprotein often found on the surface of neutrophils, hematopoietic cells, B cells and dendritic cells. CD45 is a component of vital cellular pathways, for example, the T cell receptor signaling pathway, negative regulation of cytokine-mediated signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. Also, in many organisms, it enhances hematopoietic stem cell migration, is a suppressor of cytokine-mediated signaling pathway and enhances protein tyrosine phosphatase activity. CD45 has been thought to be involved with vital biological processes such as dephosphorylation, especially protein dephosphorylation, and is associated with a variety of biologically interesting macromolecules/ligands, for example, p59fyn and Src kinases. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 510 (ex/em = 412/505 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 16).

Spectrum


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spectrum

Spectral properties

Absorbance (nm)410
Correction Factor (260 nm)0.464
Correction Factor (280 nm)0.366
Extinction coefficient (cm -1 M -1)250001
Excitation (nm)412
Emission (nm)505
Quantum yield0.861

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd45 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.