mFluor™ Violet 510 Anti-human CD8 Antibody *HIT8a*

The HIT8a monoclonal antibody binds to human CD8a, a 32 - 34 kD member of the Ig superfamily often located on the surface of dendritic cells, t cells and thymocytes. CD8 is a component of important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 510 (ex/em = 412/505 nm). It is compatible with the 405 nm laser and 528/45 nm bandpass filter (for example, as in the BD FACSVerse™).