mFluor™ Violet 540 Anti-human CD16 Antibody 3G8

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	water

Spectral properties

Absorbance (nm)	401
Correction Factor (260 nm)	1.326
Correction Factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Overview SDS Protocol

Other names

FCRIIIA

Clone

3G8

Host

mouse

Reactivity

human

Application

Flow Cytometry (FACS); Fluorescence Imaging

Molecular weight

N/A

Absorbance (nm)

401

Correction Factor (260 nm)

1.326

Correction Factor (280 nm)

0.543

Extinction coefficient (cm ^-1 M ^-1)

18000¹

Excitation (nm)

402

Emission (nm)

535

Quantum yield

0.21¹

The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. CD16 is a member of key cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like IgG Fc. CD16 is a very popular antibody target, with over 25000 publications in the last decade. CD16a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 10).

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	401
Correction Factor (260 nm)	1.326
Correction Factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21¹

Antibody Finder

Select Your CD Antibody
Select Your Clone
Select Your Conjugate

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd16a antibody with HRP). A list of available labels can be found in the table below:

AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.

Images

Figure 1. Flow cytometry analysis of whole blood cells stained with mFluor™ Violet 540 Anti-human CD16 (Clone: 3G8). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the mFluor™ Violet 540 specific V7-A channel.