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mFluor™ Violet 540 Anti-human CD19 Antibody
4G7
The 4G7 monoclonal antibody binds with human CD19, a 95 kD transmembrane protein commonly expressed on the surface of B cells and lymphocytes. CD19 plays a role in essential cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. Moreover, in some organisms, it acts to positively regulate phosphatidylinositol 3-kinase activity, is an enhancer of protein kinase B signaling and is involved in the positive regulation of release of sequestered calcium ion into cytosol. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Fyn and PI3-kinase. CD19 is a very popular antibody target, with over 30000 publications in the last decade. CD19 is essential for immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 16).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10193120 | 100 tests | Price | |
| 10193121 | 500 tests | Price |
Antibody properties
| Other names | B4 |
| Clone | 4G7 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 21, 2026