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mFluor™ Violet 540 Anti-human CD20 Antibody
HI20a
HI20a is an anti-human monoclonal antibody that recognizes the CD20 antigen. CD20 (alternatively called MS4A1, Bp35 or Leukocyte surface antigen Leu-16) is a 33 - 37 kD transmembrane protein that is expressed on the surface of cells such as B cells. CD20 plays a role in important cellular pathways, namely, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as Lyn. CD20 is a moderately popular antibody target, with over 20000 publications in the last decade. CD20 has a variety of applications in costimulatory molecules and immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 510/50 nm bandpass filter (for example, as in the BD FACSCanto™ II).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10201120 | 100 tests | Price | |
| 10201121 | 500 tests | Price |
Antibody properties
| Other names | Bp35, B1 |
| Clone | HI20a |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 13, 2025