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mFluor™ Violet 540 Anti-human CD235 Antibody
HIR2
HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen. CD235a (also known as Glycophorin A) is a 10 kD single-pass type i membrane protein that is found on the surface of cells such as stem cells and erythrocytes. CD235 is associated with a variety of biologically interesting macromolecules/ligands, in particular, Plasmodium falci- parum erythrocyte binding antigen EBA-175. CD235 is a relatively rare antibody target, with less than 0 publications in the last decade. Even still, CD235a has been widely used in cell adhesion and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 12351120 | 100 tests | Price | |
| 12351121 | 500 tests | Price |
Antibody properties
| Other names | GYPA, Sialoglycoprotein alpha, PAS-2 |
| Clone | HIR2 |
| Host | Mouse |
| Isotype | IgG2b |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 20, 2026