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mFluor™ Violet 540 Anti-human CD32 Antibody
3D3
The 3D3 monoclonal antibody binds to human CD32, a 40 kD member of the Ig superfamily often located on the surface of platelets, monocytes, B cells, granulocytes and dendritic cells. CD32 is involved with essential cellular pathways, namely, the Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands. CD32 is a fairly uncommon antibody target, with a little more than 7000 publications in the last decade. Even still, CD32 has been widely used in innate immunity and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 16).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10321120 | 100 tests | Price | |
| 10321121 | 500 tests | Price |
Antibody properties
| Other names | FcγRII, Fc gamma RII |
| Clone | 3D3 |
| Host | Mouse |
| Isotype | Mouse IgG1 kappa |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 22, 2025