Products
Services
Resources
Selection Guides
About
mFluor™ Violet 540 Anti-human CD4 Antibody
RPA-T4
RPA-T4 is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (sometimes called T4 or Leu3a) is a 55 kD transmembrane glycoprotein that is expressed on the surface of cells such as granulocytes, T cells and macrophages. CD4 acts in vital cellular pathways, for instance, the T cell receptor signaling pathway, interleukin-15-mediated signaling pathway and enzyme linked receptor protein signaling pathway. Furthermore, in many organisms, it promotes interleukin-2 biosynthetic process, upregulates peptidyl-tyrosine phosphorylation and acts to positively regulate I-kappaB kinase/NF-kappaB signaling. CD4 has been associated with key biological processes such as membrane organization, specifically fusion of virus membrane with host plasma membrane, and is associated with a variety of biologically interesting macromolecules/ligands, namely, gp120 and Lck. CD4 is a very popular antibody target, with over 180000 publications in the last decade. CD4 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD FACSCanto™).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10041120 | 100 tests | Price | |
| 10041121 | 500 tests | Price |
Antibody properties
| Other names | Leu-3, T4 |
| Clone | RPA-T4 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on April 7, 2026