Name: mFluor™ Violet 540 Anti-human CD5 Antibody *HISM2*
Brand: AAT Bioquest
SKU: 10050120
Availability: InStock

mFluor™ Violet 540 Anti-human CD5 Antibody

HISM2

HISM2 is an anti-human monoclonal antibody that forms an immune complex with the CD5 antigen. CD5 (sometimes referred to as LEU1, Tp67 or Lyt-1) is a 67 kD single-pass type I membrane protein that is located on the surface of cells like B cells and T cells. CD5 is a component of vital cellular pathways, for instance, the apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like ZAP-70, TCR and BCR. CD5 is a moderately popular antibody target, with over 11000 publications in the last decade. CD5 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSAria™ II).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
10050120	100 tests	Price
10050121	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd5 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leu1, Ly-1, T1, Tp67
Clone	HISM2
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	401
Correction factor (260 nm)	1.326
Correction factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000 ¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on February 16, 2026