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mFluor™ Violet 540 Anti-human CD52 Antibody
HI186
HI186 is an anti-human monoclonal antibody that is specific for the CD52 antigen. CD52 (also known as HE5) is a 7 kD transmembrane glycoprotein that is located on the surface of cells such as epithelial cells, NK cells and T cells. In some organisms, CD52 is an enhancer of cytosolic calcium ion concentration, and is associated with a variety of biologically interesting macromolecules/ligands, in particular, . CD52 is a fairly uncommon antibody target, with a little more than 2600 publications in the last decade. Even still, CD52 is vital to costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 525/50 nm bandpass filter (for example, as in the BD FACSCanto™).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10520120 | 100 tests | Price | |
| 10520121 | 500 tests | Price |
Antibody properties
| Other names | CAMPATH-1, HE5 |
| Clone | HI186 |
| Host | Mouse |
| Isotype | IgG2b |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 12, 2026