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mFluor™ Violet 540 Anti-human CD53 Antibody
HI36
The HI36 monoclonal antibody binds to human CD53, a 35 - 42 kD member of the tetraspanin/transmambrane-4 superfamily typically expressed on the surface of leukocytes, B cells, dendritic cells, osteoblasts and thymocytes. In many organisms, CD53 plays a role in the upregulation of myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, for instance, VLA-4 and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 is vital to costimulatory molecules research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 537/65 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10531120 | 100 tests | Price | |
| 10531121 | 500 tests | Price |
Antibody properties
| Other names | Tetraspanin-25, MOX44 |
| Clone | HI36 |
| Host | Mouse |
| Isotype | IgG3 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 12, 2026