Name: mFluor™ Violet 540 Anti-human CD57 Antibody *HI57a*
Brand: AAT Bioquest
SKU: 10570120
Availability: InStock

mFluor™ Violet 540 Anti-human CD57 Antibody

HI57a

The HI57a monoclonal antibody binds with human CD57, a 100 - 115 kD transmembrane protein often expressed on the surface of T cells, natural killer cells, granulocytes and platelets. CD57 is associated with a variety of biologically interesting macromolecules/ligands, for example, P-Selectin and Laminin. CD57 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD57 has a variety of applications in costimulatory molecules and immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 512/25 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
10570120	100 tests	Price
10570121	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD57 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Physical properties

Molecular weight	N/A
Solvent	water

Antibody properties

Other names	HNK-1, Leu-7
Clone	HI57a
Host	Mouse
Isotype	IgM
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	401
Correction factor (260 nm)	1.326
Correction factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000 ¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 12, 2026