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mFluor™ Violet 540 Anti-human CD57 Antibody *HI57a*

The HI57a monoclonal antibody binds with human CD57, a 100 - 115 kD transmembrane protein often expressed on the surface of T cells, natural killer cells, granulocytes and platelets. CD57 is associated with a variety of biologically interesting macromolecules/ligands, for example, P-Selectin and Laminin. CD57 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD57 has a variety of applications in costimulatory molecules and immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 512/25 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd57 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on May 13, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1057012010570121
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other namesHNK-1; Leu-7
CloneHI57a
Hostmouse
Isotypemouse IgM
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Physical properties

Molecular weight

N/A

Solvent

water

Spectral properties

Absorbance (nm)

401

Correction Factor (260 nm)

1.326

Correction Factor (280 nm)

0.543

Extinction coefficient (cm -1 M -1)

180001

Excitation (nm)

402

Emission (nm)

535

Quantum yield

0.211

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
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