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mFluor™ Violet 540 Anti-mouse CD49 Antibody *5H10-27 (MFR5)*

5H10-27 (MFR5) is an anti-mouse monoclonal antibody that forms an immune complex with the CD49e antigen. CD49e (also known as ITGA5, Integrin α5 chain or VLA-5α) is a 135 kD member of the Integrin family that is found on the surface of cells like stem cells and T cells. CD49 is associated with a variety of biologically interesting macromolecules/ligands, for example, fibronectin. CD49 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD49e is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 530/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd49e antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on June 30, 2025

Ordering information

Price
Unit size
100 tests
500 tests
Catalog Number
1049312010493121
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Antibody properties

Other namesVLA-5α; Integrin α5 chain; ITGA5
Clone5H10-27 (MFR5)
Hostrat
IsotypeRat IgG2a kappa
Reactivitymouse
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)

401

Correction Factor (260 nm)

1.326

Correction Factor (280 nm)

0.543

Extinction coefficient (cm -1 M -1)

180001

Excitation (nm)

402

Emission (nm)

535

Quantum yield

0.211

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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