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mFluor™ Violet 540 Anti-mouse CD49 Antibody
5H10-27 (MFR5)
5H10-27 (MFR5) is an anti-mouse monoclonal antibody that forms an immune complex with the CD49e antigen. CD49e (also known as ITGA5, Integrin α5 chain or VLA-5α) is a 135 kD member of the Integrin family that is found on the surface of cells like stem cells and T cells. CD49 is associated with a variety of biologically interesting macromolecules/ligands, for example, fibronectin. CD49 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD49e is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to mFluor™ Violet 540 (ex/em = 394/537 nm). It is compatible with the 405 nm laser and 530/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10493120 | 100 tests | Price | |
| 10493121 | 500 tests | Price |
Antibody properties
| Other names | VLA-5α, Integrin α5 chain, ITGA5 |
| Clone | 5H10-27 (MFR5) |
| Host | Rat |
| Isotype | Rat IgG2a kappa |
| Reactivity | Mouse |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Absorbance (nm) | 401 |
| Correction factor (260 nm) | 1.326 |
| Correction factor (280 nm) | 0.543 |
| Extinction coefficient (cm -1 M -1) | 18000 1 |
| Excitation (nm) | 402 |
| Emission (nm) | 535 |
| Quantum yield | 0.21 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on December 21, 2025