MMP Green™ substrate

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<p>The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity.</p>
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Unit Size: Cat No: Price (USD): Qty:
1 mg 13520 $495

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Ex/Em (nm)498/520
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Category Enzyme Detection
Peptidases and Proteases
Related Microplate Readers
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This FRET substrate is designed to monitor the general activity of a MMP enzyme. It can also be used to screening MMP inhibitors when a purified MMP enzyme is used. This FRET substrate is based on our TF2/TQ2 FRET pair. Upon MMP hydrolysis the fluorescence of MMP Green™ FRET peptide substrate is increased since the TF2/TQ2 FRET pair is separated. The fluorescence increase is proportional to the MMP enzyme activities.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of MMP Green™ substrate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =

Spectrum Advanced Spectrum Viewer

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Wavelength (nm)

References & Citations

Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway
Authors: Ruyue Xiao, Lan Yuan, Weijiang He, Xiaoda Yang
Journal: Metallomics (2018)

Connexin 43 Upregulation by Dioscin Inhibits Melanoma Progression via Suppressing Malignancy and Inducing M1 Polarization
Authors: Yu Kou, Liyan Ji, Haojia Wang, Wensheng Wang, Hongming Zheng, Juan Zou, Linxin Liu, Xiaoxiao Qi, Zhongqiu Liu, Biaoyan Du
Journal: International Journal of Cancer (2017)

DACT2, an epigenetic stimulator, exerts dual efficacy for colorectal cancer prevention and treatment
Authors: Linlin Lu, Ying Wang, Rilan Ou, Qian Feng, Liyan Ji, Hongming Zheng, Yue Guo, Xiaoxiao Qi, Ah-Ng Tony Kong, Zhongqiu Liu
Journal: Pharmacological research (2017)

Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay
Authors: Harsha Kittur, Andy Tay, Avery Hua, Min Yu, Dino Di Carlo
Journal: Biophysical Journal (2017): 1858--1867

Inhibition of BET bromodomain attenuates angiotensin II induced abdominal aortic aneurysm in ApoE-/- mice
Authors: Qiong Duan, Xiaoxiao Mao, Chaonan Liao, Haoyang Zhou, Zelin Sun, Xu Deng, Qiuning Hu, Jun Qi, Guogang Zhang, He Huang
Journal: International Journal of Cardiology (2016): 428--432

Tissue inhibitor of metalloproteinase 1 influences vascular adaptations to chronic alterations in blood flow
Authors: Erin R Mandel, Cassandra Uchida, Emmanuel Nwadozi, Armin Makki, Tara L Haas
Journal: Journal of Cellular Physiology (2016)

β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
Authors: Silvia Moretti, Daniela Massi, Valentina Farini, Gianna Baroni, Matteo Parri, Stefania Innocenti, Roberto Cecchi, Paola Chiarugi
Journal: Laboratory Investigation (2013): 279--290

Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3
Authors: Laralynne M Przybyla, Thorold W Theunissen, Rudolf Jaenisch, Joel Voldman
Journal: Stem Cells (2013): 1097--1106

Identification and functional validation of CDH11, PCSK6 and SH3GL3 as novel glioma invasion-associated candidate genes
Authors: S Delic, N Lottmann, K Jetschke, G Reifenberger, Markus J Riemenschneider
Journal: Neuropathology and applied neurobiology (2012): 201--212

EphA2 induces metastatic growth regulating amoeboid motility and clonogenic potential in prostate carcinoma cells
Authors: Maria Letizia Taddei, Matteo Parri, Adriano Angelucci, Francesca Bianchini, Chiara Marconi, Elisa Giannoni, Giovanni Raugei, Mauro Bologna, Lido Calorini, Paola Chiarugi
Journal: Molecular Cancer Research (2011): 149--160

Additional Documents

Safety Data Sheet (SDS)

1. Enzyme Probes & Assay Kits

Certificate of Analysis