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Nuclear Blue™ DCS2 *Equivalent to SYTOX™ Blue Dead Cell Stain*

Nuclear Blue™ DCS2 is the same molecule to SYTOX™ Blue Dead Cell Stain (SYTOX™ is a trademark of ThermoFisher). Nuclear Blue™ DCS2 is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. It is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. Under the same conditions, our Nuclear Violet™ DCS1 (#17549) gives much higher signal/background ratio than SYTOX™ Blue Dead Cell Stain. Nuclear Violet™ DCS1 is better excited by the violet laser at 405 nm than SYTOX™ Blue Dead Cell Stain. After brief incubation with Nuclear Violet™ DCS1 stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of Nuclear Violet™ DCS1 stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a general guideline and is adaptable for any cell type.

Note: Growth medium, cell density, and other factors may influence staining. For optimal staining, try a range of dye concentrations to determine the one that yields the best results.

Note: Before using, thaw the vial of Nuclear Blue™ DCS2 to room temperature 

  1. Harvest sample cells using an appropriate buffer and adjust the cell concentration of the sample to be from 1 x 105 to 5 x 107 cells/mL.

  2. Prepare flow cytometry tube(s) containing 1 mL of cell suspension.

  3. Add 1 µL of Nuclear Blue™ DCS2 to each flow cytometry tube.

  4. Incubate flow cytometry tubes for 5 to 10 minutes at room temperature.

  5. Analyze samples without washing on a flow cytometer with a 473/15 nm emission filter.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Nuclear Blue™ DCS1 *5 mM DMSO Solution*348469
Nuclear Blue™ LCS1353456

References

View all 50 references: Citation Explorer
Characterization of microglia behaviour in healthy and pathological conditions with image analysis tools.
Authors: Martinez, Aleix and Hériché, Jean-Karim and Calvo, Maria and Tischer, Christian and Otxoa-de-Amezaga, Amaia and Pedragosa, Jordi and Bosch, Anna and Planas, Anna M and Petegnief, Valérie
Journal: Open biology (2023): 220200
A comprehensive method to study the DNA's association with lamin and chromatin compaction in intact cell nuclei at super resolution.
Authors: Chapman, Katarina B and Filipsky, Filip and Peschke, Nicolas and Gelléri, Márton and Weinhardt, Venera and Braun, Andrejs and Hausmann, Michael and Cremer, Christoph
Journal: Nanoscale (2023): 742-756
6-C-Linked trehalose glycolipids signal through Mincle and exhibit potent adjuvant activity.
Authors: Thathsaranie P Manthrirathna, M A and Kodar, Kristel and Ishizuka, Shigenari and Dangerfield, Emma M and Xiuyuan, Lu and Yamasaki, Sho and Stocker, Bridget L and S M Timmer, Mattie
Journal: Bioorganic chemistry (2023): 106345
Asebogenin suppresses thrombus formation via inhibition of Syk phosphorylation.
Authors: Li, Li and Xu, Xulin and Lv, Keyu and Zheng, Guijuan and Wang, Hao and Chen, Shuai and Huang, Lang and Liu, Yi and Zhang, Yadong and Tang, Zhaoming and Zhang, Lili and Wang, Jinyu and Qiao, Jianlin and Li, Hongliang and Wang, Xuanbin and Yao, Guangmin and Fang, Chao
Journal: British journal of pharmacology (2023): 287-307
Effects of Imatinib Combined With Everolimus on Mouse Pituitary Tumor Cell AtT-20.
Authors: Wang, Wenxin and Lin, Yi and Qu, Xinguo and Meng, Linghu and Yang, Jianquan
Journal: Alternative therapies in health and medicine (2023): 238-244
Page updated on October 12, 2024

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Physical properties

Molecular weight

668.36

Solvent

DMSO

Spectral properties

Excitation (nm)

445

Emission (nm)

470

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

Flow cytometer

Excitation405 nm Laser
Emission473, 15 nm Filter
A mixture of heat-killed and untreated Jurkat cells was stained with Nuclear Blue™ DCS2 (17546) or Nuclear Violet™ DCS1 (17549) stain for 10 minutes. Cells were analyzed on a flow cytometer equipped with a 405 nm violet laser and a 473/15 nm bandpass filter, such as the V4 channel on Cytek's Aurora spectral flow cytometer. Live cells are easily distinguished from the dead cell population.
A mixture of heat-killed and untreated Jurkat cells was stained with Nuclear Blue™ DCS2 (17546) or Nuclear Violet™ DCS1 (17549) stain for 10 minutes. Cells were analyzed on a flow cytometer equipped with a 405 nm violet laser and a 473/15 nm bandpass filter, such as the V4 channel on Cytek's Aurora spectral flow cytometer. Live cells are easily distinguished from the dead cell population.
A mixture of heat-killed and untreated Jurkat cells was stained with Nuclear Blue™ DCS2 (17546) or Nuclear Violet™ DCS1 (17549) stain for 10 minutes. Cells were analyzed on a flow cytometer equipped with a 405 nm violet laser and a 473/15 nm bandpass filter, such as the V4 channel on Cytek's Aurora spectral flow cytometer. Live cells are easily distinguished from the dead cell population.